Simple Absolute Quantification Method Correcting for Quantitative PCR Efficiency Variations for Microbial Community Samples

Absolute quantification of target DNA: a simple competitive PCR for efficient analysis of multiple samples.

PCR is an exquisitely sensitive method for detection of minute amounts of target DNA. The exponential nature of DNA amplification, however, is prone to burden the experimental data with significant standard error due to the tube-to-tube inherent variations in amplification efficiency. Therefore the reliable quantitation of DNA or RNA templates by PCR necessitates introduction of an internal sta...

A simple, accurate and universal method for quantification of PCR.

BACKGROUND Research into gene expression enables scientists to decipher the complex regulatory networks that control fundamental biological processes. Quantitative real-time PCR (qPCR) is a powerful and ubiquitous method for interrogation of gene expression. Accurate quantification is essential for correct interpretation of qPCR data. However, conventional relative and absolute quantification m...

Evaluation of Digital PCR for Absolute RNA Quantification

Gene expression measurements detailing mRNA quantities are widely employed in molecular biology and are increasingly important in diagnostic fields. Reverse transcription (RT), necessary for generating complementary DNA, can be both inefficient and imprecise, but remains a quintessential RNA analysis tool using qPCR. This study developed a Transcriptomic Calibration Material and assessed the RT...

Evaluating efficiency of radio waves for microbial removal in water samples

The most common used methods for water disinfection were chemicals like chlorine, ozonation, ultraviolet radiation, membrane processes, etc. Water disinfection using irradiation techniques is new in water treatment industry which has been developed recently. The aim of the present study was to investigate radio frequency (RF) efficiency for the inactivation of total coliform (TC), fecal colifor...

Human Fecal Samples for Quantification of Bifidobacteria in New Real-Time Quantitative PCR Procedure